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rbs-100 ribosome binding site rbs  (GenScript corporation)

 
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    GenScript corporation rbs-100 ribosome binding site rbs
    The functionality of the <t>heterologous</t> <t>ECF</t> transcriptional system in S. venezuelae ISP5230. A , Genetic layout of the ECF transcription system. (ECF X : ECF11_3726, ECF11_987, ECF16_3622, ECF16_973, ECF34_1384, ECF27_4265, ECF38_1322 (named as ECF38), ECF17_1458 (named as ECF17); the corresponding P ecfx : P ecf11_3726 , P ecf11_987 , P ecf16_3622 , P ecf16_973 , P ecf34_1384 , P ecf27_4265 , P ecf38_1322 (named as P ecf38 ), P ecf17_1458 (named as P ecf17 ). The <t>kasO</t> p* promoter was used as a positive control. The pPAP-P ecfx plasmid in S. venezuelae ISP5230 solely was used as a negative control to exclude potential interference from the host transcription machinery. B , The activities of ECF σ factors in S. venezuelae ISP5230 were monitored by confocal fluorescence microscopy and processed by ImageJ software. C , Precise quantification of P ecf17 promoter activity using flow cytometry. Data represent the mean ± SD of at least three replicate experiments, a.u. means arbitrary unit, ** indicates significant difference.
    Rbs 100 Ribosome Binding Site Rbs, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rbs-100 ribosome binding site rbs/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    rbs-100 ribosome binding site rbs - by Bioz Stars, 2026-02
    90/100 stars

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    1) Product Images from "Novel switchable ECF sigma factor transcription system for improving thaxtomin A production in Streptomyces"

    Article Title: Novel switchable ECF sigma factor transcription system for improving thaxtomin A production in Streptomyces

    Journal: Synthetic and Systems Biotechnology

    doi: 10.1016/j.synbio.2022.05.010

    The functionality of the heterologous ECF transcriptional system in S. venezuelae ISP5230. A , Genetic layout of the ECF transcription system. (ECF X : ECF11_3726, ECF11_987, ECF16_3622, ECF16_973, ECF34_1384, ECF27_4265, ECF38_1322 (named as ECF38), ECF17_1458 (named as ECF17); the corresponding P ecfx : P ecf11_3726 , P ecf11_987 , P ecf16_3622 , P ecf16_973 , P ecf34_1384 , P ecf27_4265 , P ecf38_1322 (named as P ecf38 ), P ecf17_1458 (named as P ecf17 ). The kasO p* promoter was used as a positive control. The pPAP-P ecfx plasmid in S. venezuelae ISP5230 solely was used as a negative control to exclude potential interference from the host transcription machinery. B , The activities of ECF σ factors in S. venezuelae ISP5230 were monitored by confocal fluorescence microscopy and processed by ImageJ software. C , Precise quantification of P ecf17 promoter activity using flow cytometry. Data represent the mean ± SD of at least three replicate experiments, a.u. means arbitrary unit, ** indicates significant difference.
    Figure Legend Snippet: The functionality of the heterologous ECF transcriptional system in S. venezuelae ISP5230. A , Genetic layout of the ECF transcription system. (ECF X : ECF11_3726, ECF11_987, ECF16_3622, ECF16_973, ECF34_1384, ECF27_4265, ECF38_1322 (named as ECF38), ECF17_1458 (named as ECF17); the corresponding P ecfx : P ecf11_3726 , P ecf11_987 , P ecf16_3622 , P ecf16_973 , P ecf34_1384 , P ecf27_4265 , P ecf38_1322 (named as P ecf38 ), P ecf17_1458 (named as P ecf17 ). The kasO p* promoter was used as a positive control. The pPAP-P ecfx plasmid in S. venezuelae ISP5230 solely was used as a negative control to exclude potential interference from the host transcription machinery. B , The activities of ECF σ factors in S. venezuelae ISP5230 were monitored by confocal fluorescence microscopy and processed by ImageJ software. C , Precise quantification of P ecf17 promoter activity using flow cytometry. Data represent the mean ± SD of at least three replicate experiments, a.u. means arbitrary unit, ** indicates significant difference.

    Techniques Used: Positive Control, Plasmid Preparation, Negative Control, Fluorescence, Microscopy, Software, Activity Assay, Flow Cytometry



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    rbs-100 ribosome binding site rbs  (GenScript corporation)
    90
    GenScript corporation rbs-100 ribosome binding site rbs
    The functionality of the <t>heterologous</t> <t>ECF</t> transcriptional system in S. venezuelae ISP5230. A , Genetic layout of the ECF transcription system. (ECF X : ECF11_3726, ECF11_987, ECF16_3622, ECF16_973, ECF34_1384, ECF27_4265, ECF38_1322 (named as ECF38), ECF17_1458 (named as ECF17); the corresponding P ecfx : P ecf11_3726 , P ecf11_987 , P ecf16_3622 , P ecf16_973 , P ecf34_1384 , P ecf27_4265 , P ecf38_1322 (named as P ecf38 ), P ecf17_1458 (named as P ecf17 ). The <t>kasO</t> p* promoter was used as a positive control. The pPAP-P ecfx plasmid in S. venezuelae ISP5230 solely was used as a negative control to exclude potential interference from the host transcription machinery. B , The activities of ECF σ factors in S. venezuelae ISP5230 were monitored by confocal fluorescence microscopy and processed by ImageJ software. C , Precise quantification of P ecf17 promoter activity using flow cytometry. Data represent the mean ± SD of at least three replicate experiments, a.u. means arbitrary unit, ** indicates significant difference.
    Rbs 100 Ribosome Binding Site Rbs, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rbs-100 ribosome binding site rbs/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    rbs-100 ribosome binding site rbs - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

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    The functionality of the heterologous ECF transcriptional system in S. venezuelae ISP5230. A , Genetic layout of the ECF transcription system. (ECF X : ECF11_3726, ECF11_987, ECF16_3622, ECF16_973, ECF34_1384, ECF27_4265, ECF38_1322 (named as ECF38), ECF17_1458 (named as ECF17); the corresponding P ecfx : P ecf11_3726 , P ecf11_987 , P ecf16_3622 , P ecf16_973 , P ecf34_1384 , P ecf27_4265 , P ecf38_1322 (named as P ecf38 ), P ecf17_1458 (named as P ecf17 ). The kasO p* promoter was used as a positive control. The pPAP-P ecfx plasmid in S. venezuelae ISP5230 solely was used as a negative control to exclude potential interference from the host transcription machinery. B , The activities of ECF σ factors in S. venezuelae ISP5230 were monitored by confocal fluorescence microscopy and processed by ImageJ software. C , Precise quantification of P ecf17 promoter activity using flow cytometry. Data represent the mean ± SD of at least three replicate experiments, a.u. means arbitrary unit, ** indicates significant difference.

    Journal: Synthetic and Systems Biotechnology

    Article Title: Novel switchable ECF sigma factor transcription system for improving thaxtomin A production in Streptomyces

    doi: 10.1016/j.synbio.2022.05.010

    Figure Lengend Snippet: The functionality of the heterologous ECF transcriptional system in S. venezuelae ISP5230. A , Genetic layout of the ECF transcription system. (ECF X : ECF11_3726, ECF11_987, ECF16_3622, ECF16_973, ECF34_1384, ECF27_4265, ECF38_1322 (named as ECF38), ECF17_1458 (named as ECF17); the corresponding P ecfx : P ecf11_3726 , P ecf11_987 , P ecf16_3622 , P ecf16_973 , P ecf34_1384 , P ecf27_4265 , P ecf38_1322 (named as P ecf38 ), P ecf17_1458 (named as P ecf17 ). The kasO p* promoter was used as a positive control. The pPAP-P ecfx plasmid in S. venezuelae ISP5230 solely was used as a negative control to exclude potential interference from the host transcription machinery. B , The activities of ECF σ factors in S. venezuelae ISP5230 were monitored by confocal fluorescence microscopy and processed by ImageJ software. C , Precise quantification of P ecf17 promoter activity using flow cytometry. Data represent the mean ± SD of at least three replicate experiments, a.u. means arbitrary unit, ** indicates significant difference.

    Article Snippet: Specifically, the kasO p* promoter, rbs-100 ribosome binding site (RBS), codon-optimized ECF σ g e n e s ( T a b l e S 1 ) , a n d t w o f l a n k i n g Bsa I restriction sites were synthesized by the GenScript company, and assembled on the pTHS plasmid via Golden gate method to generate eight pTHS -ECFx plasmids ( ).

    Techniques: Positive Control, Plasmid Preparation, Negative Control, Fluorescence, Microscopy, Software, Activity Assay, Flow Cytometry